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Most home bakers get a brown shine on their pretzels by dipping the dough into a boiling bath of water and baking soda. But for Richard J. Karpowicz Jr., it’s all about the base.

“I’m a chemist, so I make traditional German lye pretzels with one molar sodium hydroxide,” he said. “I wear gloves, and I kick my girlfriend out of the kitchen.”

Richard Karpowicz Jr. developed a tag-and-quench fluorescence imaging approach to study Parkinson’s proteins. Courtesy of Richard Karpowicz Jr.

Karpowicz, a postdoctoral researcher at the University of Pennsylvania, received a 2018 Journal of Biological Chemistry/Herbert Tabor Young Investigator Award for other pursuits in chemistry. With his colleagues, Karpowicz developed a new approach to image aggregation and transmission of a protein associated with Parkinson’s disease, work that appeared in JBC last year.

Raised in Lawrenceville, New Jersey, Karpowicz went to the University of Delaware as an undergraduate intending to major in chemical engineering. His father, Richard J. Karpowicz Sr., had earned a Ph.D. in chemistry at the University of Delaware and introduced him to scientific thinking at a young age.

“I grew up doing a lot of fishing with him, and he would explain the tides and sunrise and sunset,” the younger Karpowicz said. “He always took a scientific perspective on explaining the natural world in a way that was accessible to me as a kid.”

In college, Karpowicz fell in love with biochemistry and changed his major. His research into catalyst design with Joseph Fox won him Pfizer and Howard Hughes Medical Institute funding.

Karpowicz’s interests have repeatedly pulled him from pure chemistry toward its interface with biology. In graduate school, he intended to continue with synthetic organic chemistry but heard about Dali Sames at Columbia University who develops fluorescent neurotransmitter analogues to track vesicle release.

“That’s where I got involved in tissue culture, assay development, and biological and biochemical and optics-based methodology,” he said.

After earning his Ph.D., Karpowicz wanted to develop techniques to tackle unsolved problems. He joined the lab of at the Center for Neurodegenerative Disease Research at the University of Pennsylvania.

“When I interviewed here, the most important question was, ‘How can we measure internalization of (alpha-synuclein seeds) or where they go inside the cell? How can we shed some light on what actually happens inside a neuron?’”

He pitched a plan that came to fruition in his JBC paper.

And on his cooking hobby, he reflected, “It’s fascinating what kind of flavors can develop through different cooking techniques. It’s all chemistry, right?”